Intra-assay variation (n = 3) | Inter-assay variation (n = 3) | |||
Mean CP | CV | Mean CP | CV | |
TyrA (sample) | 15.020 | 0.06% | 15.311 | 3.58% |
TyrA (control) | 20.303 | 0.09% | 20.426 | 2.98% |
PyrB (sample) | 16.031 | 2.16% | 16.289 | 3.91% |
PyrB (control) | 11.720 | 1.32% | 12.011 | 3.18% |
Gst (sample) | 14.533 | 0.65% | 14.371 | 2.26% |
Gst (control) | 14.277 | 0.11% | 13.997 | 2.62% |
cDNA input(ng) | Mean CP (n = 3) | CV% | ΔCP (cycles) | |
High cDNA input level | ||||
TyrA (sample) | 20 | 15.020 | 0.06 | +5.283 |
TyrA (control) | 20 | 20.303 | 0.09 | |
PyrB (sample) | 20 | 16.031 | 2.16 | –4.311 |
PyrB (control) | 20 | 11.720 | 1.32 | |
Gst (sample) | 16 | 14.290 | 0.73 | –0.277 |
Gst (control) | 16 | 14.013 | 0.39 | |
Gst (sample) | 20 | 14.533 | 0.65 | –0.256 |
Gst (control) | 20 | 14.277 | 0.11 | |
Gst (sample) | 24 | 14.957 | 1.29 | –0.227 |
Gst (control) | 24 | 14.730 | 1.14 | |
Low cDNA input level | ||||
TyrA (sample) | 4.0 | 17.353 | 2.16 | +5.487 |
TyrA (control) | 4.0 | 22.840 | 1.26 | |
PyrB (sample) | 4.0 | 17.667 | 0.75 | –4.277 |
PyrB (control) | 4.0 | 13.390 | 0.12 | |
Gst (sample) | 3.2 | 16.927 | 0.643 | –0.050 |
Gst (control) | 3.2 | 16.877 | 1.81 | |
Gst (sample) | 4.0 | 16.750 | 2.31 | –0.127 |
Gst (control) | 4.0 | 16.623 | 1.04 | |
Gst (sample) | 4.8 | 16.297 | 1.68 | –0.077 |
Gst (control) | 4.8 | 16.220 | 0.69 |
Gst | TyrA | PyrB |
E = 1.99 | E = 2.09 | E = 2.16 |
High cDNA input level | ΔCP = +5.283 | ΔCP = –4.311 |
80% cDNA input ΔCP = –0.277 | R = 59.476 | R = 0.04375 |
100% cDNA input ΔCP = –0.256 | R = 58.625 | R = 0.04312 |
120% cDNA input ΔCP = –0.227 | R = 57.459 | R = 0.04226 |
Mean of R | 58.45900 | 0.04304 |
Error/CV of R | 1.73% | 1.74% |
Low cDNA input level | ΔCP = +5.487 | ΔCP = –4.277 |
16% cDNA input ΔCP = –0.050 | R = 59.104 | R = 0.03844 |
20% cDNA input ΔCP = –0.127 | R = 62.102 | R = 0.04031 |
24% cDNA input DCP = –0.077 | R = 60.208 | R = 0.03914 |
Mean of R | 60.471 | 0.03930 |
Error/CV of R | 2.50% | 2.49% |